Dissecting cellular heterogeneity of Parkinson’s disease (PD) related iPS cells during aging by integrated single cell transcriptomics and imaging analysis to identify disease modifiers
Host: UNIVERSITE DU LUXEMBOURG, Luxembourg
PhD awarding institution: UNIVERSITE DU LUXEMBOURG, Luxembourg
Lead Supervisor: A Skupin
Objectives: Recently established patient derived iPS cell approaches typically neglect the effect of aging by working with freshly differentiated cells. The proposed project will address this challenge using our PD based iPS cell collection and 1) Characterizing cellular heterogeneity during differentiation and aging of personalized iPS cells by integrating microscopy with single cell RNAseq analysis; 2) Developing a bioinformatics pipeline to identify potential disease modifiers based on network analysis and imaging based validation; 3) Integrating the obtained data into a dynamic model of cell differentiation and aging in neurodegeneration.